Limited proteolysis of nitrate reductase purified from membranes of Escherichia coli.
نویسندگان
چکیده
منابع مشابه
Molybdoenzyme biosynthesis in Escherichia coli: in vitro activation of purified nitrate reductase from a chlB mutant.
All molybdoenzyme activities are absent in chlB mutants because of their inability to synthesize molybdopterin guanine dinucleotide, which together with molybdate constitutes the molybdenum cofactor in Escherichia coli. The chlB mutants are able to synthesize molybdopterin. We have previously shown that the inactive nitrate reductase present in a chlB mutant can be activated in a process requir...
متن کاملPurification and Properties of Nitrate Reductase from Escherichia coli KlZ*
The membrane-bound enzyme nitrate reductase from Escherichia coli has been solubilized and purified 112-fold. The purified enzyme appeared homogeneous by polyacrylamide gel electrophoresis and in the analytical ultracentrifuge. The molecular weight of the enzyme, as measured on an agarose column, was 720,000 and, as measured in the ultracentrifuge, was 773,600. The SO value was determined by se...
متن کاملImmunochemical localization of nitrate reductase in Escherichia coli [proceedings].
The membrane-bound nitrate reductase from Escherichiu coli functions as the terminal enzyme of the respiratory chain of the organism when it is grown anaerobically in the presence of nitrate. The enzyme consists of two types of polypeptide chain, a and 8, of approx. mol.wts. 155000 and 65000 respectively (Enoch &Lester, 1975). In conjunction with cytochrome bN%6it constitutes a proton-transloca...
متن کاملThe iron-sulfur cluster composition of Escherichia coli nitrate reductase.
Nitrate reductase from Escherichia coli has been investigated by low-temperature magnetic circular dichroism and electron paramagnetic resonance (EPR) spectroscopies, as well as by Fe-S core extrusion, to determine the Fe-S cluster composition. The results indicate approximately one 3Fe and three or four [4Fe-4S]2+,1+ centers/molecule of isolated enzyme. The magnetic circular dichroism spectra ...
متن کاملProton translocation and the respiratory nitrate reductase of Escherichia coli.
Stoicheometries and rates of proton translocation associated with respiratory reduction of NO3- have been measured for spheroplasts of Escherichia coli grown anaerobically in the presence of NO3-. Observed stoicheiometries [leads to H+/NO3- ratio; P. Mitchell (1966) Chemiosmotic Coupling in Oxidative and Photosynthetic Phosphorylation, Glynn Research, Bodmin] were approx. 4 for L-malate oxidati...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1977
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)40604-1